PandemieGrippaleV1, Main, Exploration, bibRecord, 002555

Cross-Protection of Chicken Immunoglobulin Y Antibodies against H5N1 and H1N1 Viruses Passively Administered in Mice▿

Identifieur interne : 002555 ( Main/Exploration ); précédent : 002554; suivant : 002556

Cross-Protection of Chicken Immunoglobulin Y Antibodies against H5N1 and H1N1 Viruses Passively Administered in Mice▿

Auteurs : Michael G. Wallach [Australie] ; Richard J. Webby [États-Unis] ; Fakhrul Islam [Australie] ; Stephen Walkden-Brown [Australie] ; Eva Emmoth [Suède] ; Ricardo Feinstein [Suède] ; Kjell-Olov Gronvik [Suède]

Source :

Clinical and Vaccine Immunology : CVI [ 1556-6811 ] ; 2011.

RBID : PMC:3147324

Descripteurs français

KwdFr :
- Administration par voie nasale, Animaux, Immunoglobulines (immunologie), Immunoglobulines (usage thérapeutique), Poulets, Protection croisée (immunologie), Résultat thérapeutique, Souris, Sous-type H1N1 du virus de la grippe A (immunologie), Sous-type H5N1 du virus de la grippe A (immunologie), Vaccins antigrippaux (pharmacologie).
MESH :
- immunologie : Immunoglobulines, Protection croisée, Sous-type H1N1 du virus de la grippe A, Sous-type H5N1 du virus de la grippe A.
- pharmacologie : Vaccins antigrippaux.
- usage thérapeutique : Immunoglobulines.
- Administration par voie nasale, Animaux, Poulets, Résultat thérapeutique, Souris.

English descriptors

KwdEn :
- Administration, Intranasal, Animals, Chickens, Cross Protection (immunology), Immunoglobulins (immunology), Immunoglobulins (therapeutic use), Influenza A Virus, H1N1 Subtype (immunology), Influenza A Virus, H5N1 Subtype (immunology), Influenza Vaccines (pharmacology), Mice, Treatment Outcome.
MESH :
- chemical , immunology : Immunoglobulins.
- immunology : Cross Protection, Influenza A Virus, H1N1 Subtype, Influenza A Virus, H5N1 Subtype.
- chemical , pharmacology : Influenza Vaccines.
- chemical , therapeutic use : Immunoglobulins.
- Administration, Intranasal, Animals, Chickens, Mice, Treatment Outcome.

Abstract

Influenza viruses remain a major threat to global health due to their ability to undergo change through antigenic drift and antigenic shift. We postulated that avian IgY antibodies represent a low-cost, effective, and well-tolerated approach that can easily be scaled up to produce enormous quantities of protective antibodies. These IgY antibodies can be administered passively in humans (orally and intranasally) and can be used quickly and safely to help in the fight against an influenza pandemic. In this study, we raised IgY antibodies against H1N1, H3N2, and H5N1 influenza viruses. We demonstrated that, using whole inactivated viruses alone and in combination to immunize hens, we were able to induce a high level of anti-influenza virus IgY in the sera and eggs, which lasted for at least 2 months after two immunizations. Furthermore, we found that by use of in vitro assays to test for the ability of IgY to inhibit hemagglutination (HI test) and virus infectivity (serum neutralization test), IgYs inhibited the homologous as well as in some cases heterologous clades and strains of viruses. Using an in vivo mouse model system, we found that, when administered intranasally 1 h prior to infection, IgY to H5N1 protected 100% of the mice against lethal challenge with H5N1. Of particular interest was the finding that IgY to H5N1 cross-protected against A/Puerto Rico/8/34 (H1N1) both in vitro and in vivo. Based on our results, we conclude that anti-influenza virus IgY can be used to help prevent influenza virus infection.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3147324

DOI: 10.1128/CVI.05075-11
PubMed: 21613458
PubMed Central: 3147324

Affiliations:

Le document en format XML

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<term>Immunoglobulins (immunology)</term>
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<front><div type="abstract" xml:lang="en"><p>Influenza viruses remain a major threat to global health due to their ability to undergo change through antigenic drift and antigenic shift. We postulated that avian IgY antibodies represent a low-cost, effective, and well-tolerated approach that can easily be scaled up to produce enormous quantities of protective antibodies. These IgY antibodies can be administered passively in humans (orally and intranasally) and can be used quickly and safely to help in the fight against an influenza pandemic. In this study, we raised IgY antibodies against H1N1, H3N2, and H5N1 influenza viruses. We demonstrated that, using whole inactivated viruses alone and in combination to immunize hens, we were able to induce a high level of anti-influenza virus IgY in the sera and eggs, which lasted for at least 2 months after two immunizations. Furthermore, we found that by use of <italic>in vitro</italic>
 assays to test for the ability of IgY to inhibit hemagglutination (HI test) and virus infectivity (serum neutralization test), IgYs inhibited the homologous as well as in some cases heterologous clades and strains of viruses. Using an <italic>in vivo</italic>
 mouse model system, we found that, when administered intranasally 1 h prior to infection, IgY to H5N1 protected 100% of the mice against lethal challenge with H5N1. Of particular interest was the finding that IgY to H5N1 cross-protected against A/Puerto Rico/8/34 (H1N1) both <italic>in vitro</italic>
 and <italic>in vivo</italic>
. Based on our results, we conclude that anti-influenza virus IgY can be used to help prevent influenza virus infection.</p>
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Serveur d'exploration sur les pandémies grippales

Cross-Protection of Chicken Immunoglobulin Y Antibodies against H5N1 and H1N1 Viruses Passively Administered in Mice▿

Cross-Protection of Chicken Immunoglobulin Y Antibodies against H5N1 and H1N1 Viruses Passively Administered in Mice▿

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur les pandémies grippales
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